Crystal structure of the Autoinducer-2-bound form of Vibrio harveyi LuxP complexed with the periplasmic domain of LuxQ

Ligand-induced asymmetry in histidine sensor kinase complex regulates quorum sensing.

Autoinducer 2-binding periplasmic protein luxP

Autoinducer 2 sensor kinase/phosphatase luxQ

1:1 mixture of protein (20 mg/ml in 20 mM Tris-HCl (pH 7.5), 150 mM NaCl) and well solution (27.5% (w/v) PEG 400, 100 mM potassium nitrate, 100 mM 2-morpholinoethanesulfonic acid monohydrate (pH 6.0), 100 M 4,5-dihydroxy-2,3-pentane dione, and 1 mM boric acid), VAPOR DIFFUSION, SITTING DROP, temperature 296K

Crystal Structure of the Apo Form of Vibrio Harveyi LuxP Complexed with the Periplasmic Domain of LuxQ

Crystal Structure of LuxP from Vibrio Harveyi Complexed with Autoinducer-2

SEQUENCE
THE TWO HETEROLOGOUS LUXQ N-TERMINAL RESIDUES (GLY-SER)
ARE DERIVED FROM THE THROMBIN CLEAVAGE SIGNAL.
LUXP RESIDUES 22-26 ARE DELETED.

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS

Autoinducer 2-binding periplasmic protein luxP, Autoinducer 2 sensor kinase/phosphatase luxQ (E.C.3.1.3.-)

PERIPLASMIC BINDING PROTEIN, PER/ARNT/SIMPLE-MINDED (PAS) FOLD, AUTOINDUCER-2 (AI-2), QUORUM SENSING, HISTIDINE SENSOR KINASE, SIGNALING PROTEIN