Structure of two fungal beta-1,4-galactanases: searching for the basis for temperature and pH optimum.

4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID

2-N-ACETYL-BETA-D-GLUCOSE(RESIDUE 601) LINKED TO ASN 111 IN THE FOUR MOLECULES

MYCELIOPHTHORA THERMOPHILA IS THE ANAMORPH NAME WHILST THIELAVIA HETEROTHALLIC IS THE TELEOMORPH NAME

30% PEG4000, 0.2 M AMMONIUM SULPHATE, 0.1M HEPES PH=7.5, pH 7.50

STRUCTURE OF TWO FUNGAL BETA-1,4- GALACTANASES: SEARCHING FOR THE BASIS FOR TEMPERATURE AND PH OPTIMUM.

SHEET
DETERMINATION METHOD: DSSP
THE SHEETS PRESENTED AS "AA" IN EACH CHAIN ON SHEET RECORDS
BELOW IS ACTUALLY AN 9-STRANDED BARREL THIS IS REPRESENTED BY
A 10-STRANDED SHEET IN WHICH THE FIRST AND LAST STRANDS
ARE IDENTICAL.

THE SHEETS PRESENTED AS "BA" IN EACH CHAIN ON SHEET RECORDS
BELOW IS ACTUALLY AN 8-STRANDED BARREL THIS IS REPRESENTED BY
A 9-STRANDED SHEET IN WHICH THE FIRST AND LAST STRANDS
ARE IDENTICAL.

THE SHEETS PRESENTED AS "CA" IN EACH CHAIN ON SHEET RECORDS
BELOW IS ACTUALLY AN 8-STRANDED BARREL THIS IS REPRESENTED BY
A 9-STRANDED SHEET IN WHICH THE FIRST AND LAST STRANDS
ARE IDENTICAL.

THE SHEETS PRESENTED AS "DA" IN EACH CHAIN ON SHEET RECORDS
BELOW IS ACTUALLY AN 7-STRANDED BARREL THIS IS REPRESENTED BY
A 8-STRANDED SHEET IN WHICH THE FIRST AND LAST STRANDS
ARE IDENTICAL.

BETA-1, 4-GALACTANASES, FAMILY 53 GLYCOSIDE HYDROLASE, THERMOSTABILITY, PH OPTIMUM, CLAN GH-A, THERMOPHILE, ALKALOPHILE, HYDROLASE