Reaction centre from Rhodobacter sphaeroides strain R-26.1 complexed with brominated phosphatidylcholine

DIPHOSPHATIDYL GLYCEROL; BIS-(1,2-DIACYL-SN-GLYCERO-3-PHOSPHO)-1',3'-SN-GLYCEROL

(7R,14S)-14,15-DIBROMO-4-HYDROXY-N,N,N-TRIMETHYL-9-OXO-7-[(PALMITOYLOXY)METHYL]-3,5,8-TRIOXA-4-PHOSPHAHEXACOSAN-1-AMINIUM 4-OXIDE

Brominated Lipids Identify Lipid Binding Sites on the Surface of the Reaction Center from Rhodobacter sphaeroides.

Photosynthetic reaction center L subunit

Photosynthetic reaction center M subunit

Photosynthetic reaction center H subunit

Strain R-26.1 of Rhodobacter sphaeroides bacteria is a partial revertant of the R-26 chemical mutant of the wild-type strain 2.4.1. While R-26 has no LH2 antenna and no carotenoid, the R-26.1 has altered LH2 antenna and no carotenoid. Reaction center from R-26.1 strain is therefore identical with the wild-type strain 2.4.1 except for the missing carotenoid.

Potassium phosphate, LDAO, 1,2,3-heptanetriol, dioxane, Tris-HCl, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 16.0K

Hydrogens have been added in the riding positions; the above average isotropic B value is an average residual B after TLS refinement while the final average atomic B is 53.5
TLS thermal mode followed by the restrained refinement of atomic coordinates and isotropic B-factors; all details in the pdb-file
Unpublished structure of reaction centre at 1.95A resolution

Reaction center protein L chain, Reaction center protein M chain, Reaction center protein H chain

PHOTOSYNTHESIS, PHOTOSYNTHETIC REACTION CENTER, LIPID BINDING SITES, BROMINATED LIPIDS, MEMBRANE PROTEIN, PHOTOSYNTHESIS-MEMBRANE PROTEIN COMPLEX