Crystal structure of GAD1 from Arabidopsis thaliana

(2S)-2-amino-6-[[3-hydroxy-2-methyl-5-(phosphonooxymethyl)pyridin-4-yl]methylideneamino]hexanoic acid

A common structural basis for pH- and calmodulin-mediated regulation in plant glutamate decarboxylase.

2 uL protein solution (1.9 mg/ml Gad1, 50 mM Hepes pH 7.2, 150 mM NaCl and 10 uM PLP) + 1 uL reservoir solution (100 mM Na acetate pH 5.5, 720 mM Na formate, 9 % PEG 8000 and 9 % PEG 1000), VAPOR DIFFUSION, SITTING DROP, temperature 293K

Glutamate decarboxylase 1 (E.C.4.1.1.15)

Calmodulin-binding, Decarboxylase, Lyase, Pyridoxal phosphate

chain A and (resseq 12:111 or resseq 117:148 or resseq 150:255 or resseq 260:276 or resseq 278:299 or resseq 315:404 or resseq 415:447 )

chain B and (resseq 12:111 or resseq 117:148 or resseq 150:255 or resseq 260:276 or resseq 278:299 or resseq 315:404 or resseq 415:447 )

chain C and (resseq 12:111 or resseq 117:148 or resseq 150:255 or resseq 260:276 or resseq 278:299 or resseq 315:404 or resseq 415:447 )

chain D and (resseq 12:111 or resseq 117:148 or resseq 150:255 or resseq 260:276 or resseq 278:299 or resseq 315:404 or resseq 415:447 )

chain E and (resseq 12:111 or resseq 117:148 or resseq 150:255 or resseq 260:276 or resseq 278:299 or resseq 315:404 or resseq 415:447 )

chain F and (resseq 12:111 or resseq 117:148 or resseq 150:255 or resseq 260:276 or resseq 278:299 or resseq 315:404 or resseq 415:447 )