Crystal structure of GH family 64 laminaripentaose-producing beta-1,3-glucanase from Paenibacillus barengoltzii complexed with laminarihexaose.

The recognition mechanism of triple-helical beta-1,3-glucan by a beta-1,3-glucanase

Escherichia coli 'BL21-Gold(DE3)pLysS AG'

Se-derivatized PbBgl64A was prepared and concentrated to 15 mg ml-1 in buffer D contain 5 mM DL-Dithiothreitol (DTT), and optimized crystals were grown in drops containing 2 microliter of protein solution and 0.5 microliter of reservoir solution (14mM sodium cholate, 1.1M di-Ammonium Tartrate pH 7.0) at 293 K

AUTHORS STATE THAT THE GENEBANK ACCESSION NUMBER IS KU363233 FOR THIS SEQUENCE. The SEQUENCE HAS A MUTATION, E236A.

beta-1, 3-glucan recognition, glycoside hydrolase family 64, 3-glucanase, GH64-TLP-SF superfamily, HYDROLASE