Crystal structure of mRojoA mutant - T16V -P63F - W143A - L163V

{(4Z)-2-[(1S)-1-amino-3-(methylsulfanyl)propyl]-4-[(4-hydroxyphenyl)methylidene]-5-oxo-4,5-dihydro-1H-imidazol-1-yl}acetic acid

Brighter Red Fluorescent Proteins by Rational Design of Triple-Decker Motif.

THIS SEQUENCE WAS BASED ON THE PAMCHERRY1 PROTEIN (UNP REFERENCE D1MPT3). RESIDUES MET 64, TYR 65, AND GLY 68 form a CHROMOPHORE residue CH6.

This is a new engineered protein for which the starting point is also an engineered protein. Namely, it was generated using the sequence corresponding to the structure 3NEZ. The mutations listed in the title refer to the mutations compared to 3NEZ.

quantum yield, protein engineering, FLUORESCENT PROTEIN