Crystal structure of the trehalulose synthase MUTB in complex with trehalulose

Insights into product binding in sucrose isomerases from crystal structures of MutB from Rhizobium sp.

TREHALULOSE SYNTHASE MUTB, UNP residues 28-584

12% PEG20000, 0.1M Na cacodylate, 0.01M L-cysteine, pH 6, VAPOR DIFFUSION, HANGING DROP, temperature 292K

Trehalulose synthase, MutB, in complex with Tris (P1 form)

AN INACTIVE MUTANT OF MUTB IN COMPLEX WITH SUCROSE

AN INACTIVE MUTANT OF MUTB IN COMPLEX WITH GLUCOSE

MUTB F164L MUTANT FROM CRYSTALS SOAKED WITH GLUCOSE

MUTB F164L MUTANT FROM CRYSTALS SOAKED WITH SUCROSE

MUTB F164L MUTANT FROM CRYSTALS SOAKED WITH TREHALULOSE

MUTB F164L MUTANT FROM CRYSTALS SOAKED WITH ISOMALTULOSE

MUTB R284C MUTANT FROM CRYSTALS SOAKED WITH DEOXYNOJIRIMYCIN

Trehalulose synthase, MutB, in complex with Tris (P21 form)

MutB inactive double mutant D200A-D415N in complex with glucose

MUTB INACTIVE DOUBLE MUTANT D200A-D415N SOAKED WITH SUCROSE AND HAVING AS BOUND LIGANDS SUCROSE IN MOLECULE A AND THE REACTION PRODUCT TREHALULOSE IN MOLECULE B

NATIVE ENZYME, TIM-BARREL, (BETA/ALPHA)8, SUCROSE ISOMERASE, GLYCOSIDE HYDROLASE, TREHALULOSE SYNTHASE, GH13 FAMILY (CAZY DATABASE), CALCIUM BINDING, ISOMERASE