Crystal Structure of a Redshifted Mutant (K83M) of the Red Fluorescent Protein dRFP583/dsRed

[2-(3-CARBAMOYL-1-IMINO-PROPYL)-4-(4-HYDROXY-BENZYLIDENE)-5-OXO-4,5-DIHYDRO-IMIDAZOL-1-YL]-ACETIC ACID

Novel Chromophores and Buried Charges Control Color in mFruits(,).

0.1M HEPES, 45%MPD dissolved in 1:1 ratio by volume of ~15mg/ml protein, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K

Crystal structure of dsred, a red fluorescent protein from Discosoma sp.

Crystal structure of mStrawberry at pH 9.5

Crystal structure of mStrawberry at pH 10.5

SEQUENCE
The residues Gln 66, Tyr 67 and Gly 68 constitute the
chromophore CRQ

The biological assembly is a tetramer generated by the operations: -x+1, y, -z+3/2 for chain A and B; or -0.5x+0.866y, 0.866x+0.5y, -z+5/3 for chain C and D.

beta barrel, fluorescent, red shift, GFP, dsRED, bioluminescence, LUMINESCENT PROTEIN