4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID

Crystal Structure of Glycogen Synthase Kinase 3Beta . Structural Basis for Phosphate-Primed Substrate Specificity and Autoinhibition

CRYSTAL WERE GROWN BY THE HANGING DROP METHOD. 1UL OF PROTEIN SOLUTION (4MG/ML IN 20MM HEPES-NAOH, 500MM NACL, 2MM MGCL2, 1MM DTT, PH 7.2) WAS MIXED WITH 1UL PRECIPITANT (6% PEG8000, 100MM TRIS-HCL, PH 7.5)

SHEET
DETERMINATION METHOD: DSSP
THE SHEETS PRESENTED AS "AA" AND "BA" ON SHEET RECORDS
BELOW ARE ACTUALLY A 6-STRANDED BARREL THIS IS REPRESENTED BY
A 7-STRANDED SHEET IN WHICH THE FIRST AND LAST STRANDS
ARE IDENTICAL.

GLYCOGEN SYNTHASE KINASE-3 BETA (E.C.2.7.1.37)