Crystal Structure of the GatD/MurT Enzyme Complex from Staphylococcus aureus with bound AMPPNP

PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER

Structural basis of cell wall peptidoglycan amidation by the GatD/MurT complex of Staphylococcus aureus.

Mur ligase middle domain protein,UDP-N-acetylmuramate--alanine ligase,MurT

100 mM Tris pH 9.1
350 mM MgCl2
18 % PEG 8000
13 % Glycerol

Affinity purification was carried out with only one component affinity tagged. No conditions could be found that lead to complex disassembly. See entry 6GS2

The scattering profile is consistent with the size and shape of the heterodimer. Simulated scattering profiles for larger assemblies, such as the entire content of the ASU do not match experimental data. See entry 6GS2

Glutamine amidotransferase, UDP-N-acetylmuramate--alanine ligase

cell wall biosynthesis, peptidoglycan, antibiotic resistance, BIOSYNTHETIC PROTEIN