Leucyl-tRNA synthetase from Thermus thermophilus complexed with a sulphamoyl analogue of leucyl-adenylate

[(2R,3S,4R,5R)-5-(6-AMINO-9H-PURIN-9-YL)-3,4-DIHYDROXYTETRAHYDRO-2-FURANYL]METHYL SULFAMATE

The 2A Structure of Leucyl-tRNA Synthetase and its Complex with a Leucyl-Adenylate Analogue

Crystallization and Preliminary Crystallographic Analysis of Thermus Thermophilus Leucyl-tRNA Synthetase and its Complexes with Leucine and a Non-Hydrolysable Leucyl-Adenylate Analogue

ENZYME CONTAINS 878 RESIDUES AND TWO ZINC ATOMS

PROTEIN 10 MG/ML IN 50MM BIS-TRIS PROPANE BUFFER PH 6.0-6.5 2MM DTT, 20MM MGCL2, 1MM NAN3, 18% AMMONIUM SULPHATE EQUILBRIATED AGAINST 30-38% AMMONIUM SULPHATE IN 100MM BIS-TRIS PROPANE BUFFER PH 6.0-6.5. SEE REFERENCE 2

SHEET
THE SHEET STRUCTURE OF THIS MOLECULE IS BIFURCATED. IN
ORDER TO REPRESENT THIS FEATURE IN THE SHEET RECORDS BELOW,
TWO SHEETS ARE DEFINED.

THE C-TERMINAL DOMAIN, RESIDUES 815-878, ARE DISORDERED IN THE CRYSTAL.

SIDE-CHAINS ATOMS WITH NO ELECTRON DENSITY HAVE ZERO OCCUPANCY

AMINOACYL-TRNA SYNTHETASE, CLASS I AMINOACYL-TRNA SYNTHETASE, ATP + L-LEUCINE + TRNA (LEU) -> AMP + PPI + L-LEUCYL-TRNA(LEU), LIGASE