Crystal structure of silkworm PIWI-clade Argonaute Siwi bound to piRNA

The N-terminal region (residues 1-129) was truncated by thermolysin treatment.

The bound RNAs are co-purified endogenous piRNAs, which have divergent sequences and lengths.

50mM potassium phosphate monobasic, 20% PEG8000

CRISPR-associated endonuclease Cpf1/RNA Complex