Crystal structure of Dephospho-CoA kinase (EC 2.7.1.24) (Dephosphocoenzyme A kinase) (tm1387) from THERMOTOGA MARITIMA at 2.60 A resolution

Joint Center for Structural Genomics (JCSG)

Bent cylindrical Si-mirror (Rh coating) Diamond(111) double-bounce monochromator

30.0M NaCl, 32.5% PEG-8000, 0.1M Na,K-Phosphate pH 5.2, VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 293K

BIOMOLECULE: 1, 2, 3, 4, 5, 6, 7, 8
THIS ENTRY CONTAINS THE CRYSTALLOGRAPHIC ASYMMETRIC UNIT
WHICH CONSISTS OF 8 CHAIN(S). SEE REMARK 350 FOR
INFORMATION ON GENERATING THE BIOLOGICAL MOLECULE(S).
SIZE EXCLUSION CHROMATOGRAPHY WITH STATIC LIGHT SCATTERING
SUPPORTS THE ASSIGNMENT OF A MONOMER AS A BIOLOGICALLY
SIGNIFICANT OLIGOMERIZATION STATE.

SEQUENCE:
THE CONSTRUCT WAS EXPRESSED WITH A PURIFICATION
TAG MGSDKIHHHHHH

1. HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS. 2. THERE IS
A DEPHOSPHO-COA MOLECULE AND AN ADP MOLECULE BOUND IN EACH MONOMER.
3. FOUR CHLORIDE IONS FROM CRYSTALLIZATION SOLUTION HAVE BEEN
INCLUDED IN THE MODEL. 4. ATOM RECORD CONTAINS RESIDUAL B FACTORS ONLY.

SIZE EXCLUSION CHROMATOGRAPHY DATA SUPPORTS THE ASSIGNMENT OF A MONOMER AS A BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE.

tm1387, Dephospho-CoA kinase, EC 2.7.1.24, Dephosphocoenzyme A kinase, Structural Genomics, Joint Center for Structural Genomics, JCSG, Protein Structure Initiative, PSI, TRANSFERASE