Structure of apurinic/apyrimidinic DNA lyase TK0353 from Thermococcus kodakarensis (Selenomethionine)

2-Gaussian fit: Grosse-Kunstleve RW, Sauter NK, Adams PD: Newsletter of the IUCr Commission on Crystallographic Computing 2004, 3, 22-31.

Thermococcus kodakarensis TK0353 is a novel AP lyase with a new fold

2.5 M (NH4)2SO4 with 0.1 M Tris pH 8.0 mixed 1:1 with Protein at 20 mg/ml in 10 mM Tris pH 7.5, 100 mM NaCl, 10% glycerol and 1 mM DTT. Cryoprotection by addition of 1.5 M Li2SO4

National Institutes of Health/National Cancer Institute (NIH/NCI)

chain 'A' and (resid 1 through 9 )

chain 'A' and (resid 10 through 59 )

chain 'A' and (resid 60 through 70 )

chain 'A' and (resid 71 through 87 )

chain 'A' and (resid 88 through 108 )

chain 'A' and (resid 109 through 139 )

chain 'A' and (resid 140 through 168 )

chain 'B' and (resid 1 through 9 )

chain 'B' and (resid 10 through 59 )

chain 'B' and (resid 60 through 70 )

chain 'B' and (resid 71 through 87 )

chain 'B' and (resid 88 through 108 )

chain 'B' and (resid 109 through 139 )

chain 'B' and (resid 140 through 168 )

25 angstrom radius determined by dynamic light scattering equivalent to the monomeric species.

apurinic/apyrimidinic DNA lyase DNA binding protein, LYASE

(chain "A" and (resid 2 through 5 or (resid 6 and (name N or name CA or name C or name O or name CB )) or resid 7 through 22 or resid 24 through 26 or (resid 27 and (name N or name CA or name C or name O or name CB )) or resid 28 through 31 or resid 33 through 36 or resid 38 through 39 or resid 41 through 42 or (resid 44 and (name N or name CA or name C or name O or name CB )) or resid 45 through 46 or (resid 47 and (name N or name CA or name C or name O or name CB )) or resid 48 through 49 or (resid 50 through 51 and (name N or name CA or name C or name O or name CB )) or resid 52 through 53 or (resid 54 through 55 and (name N or name CA or name C or name O or name CB )) or resid 56 or (resid 57 and (name N or name CA or name C or name O or name CB )) or resid 59 through 60 or resid 62 through 75 or (resid 76 and (name N or name CA or name C or name O or name CB )) or resid 77 or resid 79 through 81 or resid 83 through 87 or (resid 88 and (name N or name CA or name C or name O or name CB )) or resid 89 through 158 or resid 160 through 165 or (resid 166 through 167 and (name N or name CA or name C or name O or name CB ))))

(chain "B" and (resid 2 through 22 or resid 24 through 31 or resid 33 through 36 or resid 38 through 39 or resid 41 through 42 or resid 44 through 46 or (resid 47 and (name N or name CA or name C or name O or name CB )) or resid 48 through 49 or (resid 50 through 51 and (name N or name CA or name C or name O or name CB )) or resid 52 through 53 or (resid 54 through 55 and (name N or name CA or name C or name O or name CB )) or resid 56 or (resid 57 and (name N or name CA or name C or name O or name CB )) or resid 59 through 60 or resid 62 through 77 or resid 79 through 81 or resid 83 through 114 or (resid 115 and (name N or name CA or name C or name O or name CB )) or resid 116 through 158 or resid 160 through 167))