Crystal structure of the Archaeoglobus fulgidus oligosaccharyltransferase (O29867_ARCFU) tethered with an acceptor peptide containing the NVT sequon via a disulfide bond

Tethering an N-Glycosylation Sequon-Containing Peptide Creates a Catalytically Competent Oligosaccharyltransferase Complex

Transmembrane oligosaccharyl transferase, putative

acceptor peptide, ARG-TYR-ASN-VAL-THR-ALA-CYS

10 mM MgCl2, 0.1 M Bis-Tris, pH 6.5, 22 % (w/v) PEG 550MME, 5% (v/v) Jeffamine M-600

We moved the position of the bound Mg2+ ion to a proper place and refined the whole structure again. This is an important correction because this metal ion is located at the center of the catalytic site.

Japan Society for the Promotion of Science (JSPS)

Transmembrane oligosaccharyl transferase, putative (E.C.2.4.99.18), acceptor peptide, ARG-TYR-ASN-VAL-THR-ALA-CYS

Oligosaccharyltransferase, N-lineked glycosylation, AglB, Archaeoglobus fulgidus, TRANSFERASE