Dpo4 extension ternary complex with oxoG(syn)-A(anti) and oxoG(anti)-A(syn) pairs

Impact of conformational heterogeneity of OxoG lesions and their pairing partners on bypass fidelity by Y family polymerases.

6144 x 6144 pixel elements with an effective pixel size less than 60x60 micron and resolution of order 90x90 micron

PRIMER STRAND (DIDEOXY-TERMINATED AT 3'-END)

100 mM HEPES, 100 mM Calcium acetate, 10% PEG 4000, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K

oxoG-modified Dpo4 insertion ternary complex

oxoG-modified Dpo4 pre-insertion binary complex

oxoG-modified Dpo4 post-insertion binary complex

Dpo4 extension ternary complex with disordered A opposite an oxoG in anti conformation

Dpo4 extension ternary complex with the oxoG(anti)-C(anti) pair

Dpo4 extension ternary complex with oxoG(anti)-T(anti) pair

Dpo4 extension ternary complex with oxoG(anti)-G(syn) pair

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS

DNA POLYMERASE, 8-OXOGUANINE, Y-FAMILY, LESION BYPASS, DNA damage, DNA repair, DNA replication, DNA-binding, DNA-directed DNA polymerase, Magnesium, Metal-binding, Mutator protein, Nucleotidyltransferase, Transferase, TRANSFERASE-DNA COMPLEX