Crystal Structure of Calicheamicin Methyltransferase, CalS11

Enzyme Discovery for Natural Product Biosynthesis (NatPro)

Center for Eukaryotic Structural Genomics (CESG)

Protein solution (11 mg/ml CalS11, 25mM Tris pH 8.0, 5 mg/ml calicheamicin) mixed in a 1:1 ratio with the well solution (25% PEG 3350, .2M LiSO4, .1M Tris pH 8.5) Cryoprotected with 30% PEG 3350, .2M LiSO4, .1M Tris pH 8.5, Vapor diffusion, hanging drop, temperature 298K

Center for Eukaryotic Structural Genomics

Enzyme Discovery for Natural Product Biosynthesis

Another structure of CalS11 solved by the same group under different crystallization conditions, with a protein sample prepared using cell free expression systems.

The biological unit is a decamer. There are 2 biological units in the asymmetric unit (chains A-J and chains K-T)
CalS11, SAH, Methyltransferase, Calicheamicin, Structural Genomics, Protein Structure Initiative, PSI, PSI-Biology, NATPRO, Enzyme Discovery for Natural Products Biosynthesis, Center for Eukaryotic Structural Genomics, CESG, Transferase, Enzyme Discovery for Natural Product Biosynthesis