E. coli thymidylate synthase Y209W variant in complex with substrate and a cofactor analog

A remote mutation affects the hydride transfer by disrupting concerted protein motions in thymidylate synthase.

The role of protein dynamics in thymidylate synthase catalysis: variants of conserved 2'-deoxyuridine 5'-monophosphate (dUMP)-binding Tyr-261.

vapor diffusion against 1.44 M Sodium citrate, HEPES from 5.2 mg/ml protein solution containing 17mM kPO4, DTT, 3.3 mM dUMP, 3.3 mM CB3717 and either 0.5% ethylacetate or 4% N-propanol, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 295K

E. coli Y209W thymidylate synthase apo enzyme

wild type E. coli thymidylate synthase in complex with dUMP and CB3717

L. casei thymidylate synthase Y261F-dUMP

L. casei thymidylate synthase Y261A-dUMP

L. casei thymidylate synthase Y261M-dUMP

beta sheet, alpha/beta protein, methylase, TRANSFERASE