Atomic resolution structure of cholesterol oxidase @ pH 9.0 (Streptomyces sp. SA-COO)

Atomic resolution crystallography reveals how changes in pH shape the protein microenvironment.

FAD COFACTOR NON-COVALENTLY BOUND TO THE ENZYME

PEG 8000, AMMONIUM SULFATE, PIPES PH 7.5,VAPOR DIFFUSION, HANGING DROP, temperature 298K,
SOAK CONDITION STEP 1: PEG 8000, AMMONIUM SULFATE, HEPES PH 8.0
SOAK CONDITION STEP 2: PEG 8000, AMMONIUM SULFATE, TRICINE PH 8.5
SOAK CONDITION STEP 3: PEG 8000, AMMONIUM SULFATE, CHES PH 9.0,

SEQUENCE
THE SEQUENCE OF MATURE CHOLESTEROL OXIDASE ENZYME
RUNS FROM RESIDUES 43 - 546 IN THE SEQUENCE DATABASE.
THE NUMBERING OF THE RESIDUES DIFFERS FROM THAT IN THE
DATABASE DUE TO THIS DESCREPANCY IN THE SEQUENCE. IN
ADDITION, THE NUMBERING HAS BEEN ADJUSTED TO CONFORM TO
THAT FOUND IN THE STRUCTURE OF CHOLESTEROL OXIDASE FROM
BREVIBACTERIUM STEROLICUM (ACCESSION CODE 3COX). THIS
CHANGE IN THE NUMBERING SCHEME FACILITATES EASY COMPARISON
OF THE TWO STRUCTURES.

FLAVOENZYME, STEROID METABOLISM, OXIDOREDUCTASE, ATOMIC RESOLUTION, HYDROGEN BOND