Crystal structure of human choline kinase alpha in complex with hemicholinium-3 and ADP

Crystal structures of human choline kinase isoforms in complex with hemicholinium-3: single amino acid near the active site influences inhibitor sensitivity.

25% PEG-3350, 0.2M lithium sulfate, 0.1M HEPES. Crystallization sample buffer: 0.01M TRIS pH 8.0, 0.5M sodium chloride, 0.005M magnesium chloride, 0.01M DTT, 0.003M hemicholinium-3, 0.005M ADP., vapor diffusion, temperature 291K

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS. HEMICHOLINIUM-3 RESTRAINTS WERE PREPARED BY THE PRODRG SERVER.

NON-PROTEIN KINASE, CHOLINE KINASE, STRUCTURAL GENOMICS Consortium, SGC, hemicholinium-3, Kinase, Transferase