Crystal structure of a V2-directed, RV144 vaccine-like antibody from HIV-1 infection, CAP228-16H, bound to a heterologous V2 peptide

V2-Directed Vaccine-like Antibodies from HIV-1 Infection Identify an Additional K169-Binding Light Chain Motif with Broad ADCC Activity.

Large diamond shaped crystals (0.3-0.5mm)

20% PEG8000, 200mM sodium chloride, 100mM sodium phosphate-citrate (pH4.2), 160mM ammonium sulphate, 8% 2-Methyl-2,4-pentanediol (25% 2-Methyl-2,4-pentanediol for cryoprotection)

Chains H and L were isolated from the same B cell, which was selected for based on reactivity with chain P.

Chains I and M were isolated from the same B cell, which was selected for based on reactivity with chain A.

CAP228-16H Heavy Chain, CAP228-16H Light Chain, CAP45 V2 peptide