Calcium-dependent phosphoinositol-specific phospholipase C from a Gram-negative bacterium, Pseudomonas sp, apo form, myoinositol complex

The structure of a calcium-dependent phosphoinositide-specific phospholipase C from Pseudomonas sp. 62186, the first from a Gram-negative bacterium.

PHOSPHOINOSITOL-SPECIFIC PHOSPHOLIPASE C

PEG3350 24-27%, CAPS/BICINE MIX PH 9.0-10 GRID, BEST CRYSTALS CLOSER TO PH 10, SEEDING FROM PACT H4 (0.2M KSCN, 20% PEG3350, BIS TRIS PROPANE PH 8.5), ORYX ROBOT, 24 WELL PLATE, HANGING DROPS

CALCIUM-DEPENDENT PHOSPHOINOSITOL-SPECIFIC PHOSPHOLIPASE C FROM A GRAM-NEGATIVE BACTERIUM, PSEUDOMONAS SP, APO FORM, CRYSTAL FORM 1

CALCIUM-DEPENDENT PHOSPHOINOSITOL-SPECIFIC PHOSPHOLIPASE C FROM A GRAM-NEGATIVE BACTERIUM, PSEUDOMONAS SP, APO FORM, CRYSTAL FORM 2

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS. U VALUES REFINED INDIVIDUALLY

HYDROLASE, PI-PLC, BACTERIAL, PSEUDOMONAS, GRAM-NEGATIVE, CALCIUM-DEPENDENT, MYOINOSITOL COMPLEX