human beta tryptase II complexed with activated ketone inhibitor CRA-29382

ALLYL {(1S)-1-[(5-{4-[(2,3-DIHYDRO-1H-INDEN-2-YLAMINO)CARBONYL]BENZYL}-1,2,4-OXADIAZOL-3-YL)CARBONYL]-3-PYRROLIDIN-3-YLPROPYL}CARBAMATE

Structure-guided design of Peptide-based tryptase inhibitors.

2mg/mL protein, 10 mM MES, pH 6.1, 2M NaCl dissolved in 0.1 M NaOAc, pH 4.6, 0.2 M ammonium sulfate, 30% PEG 1500. Crystallization drops were set up using various ratios of protein solution to crystallization solution. Crystals appropriate for diffraction studies appeared in 2-5 days at room temperature, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K

Crystal structure of human beta-tryptase II complexed with 4-piperidinebutyrate to make acylenzyme

Crystal structure of bovine trypsin bound by 4-piperidinebutyrate to make acylenzyme complex

Crystal structure of bovine trypsin complexed with 2-aminobenzamidazole

The biological unit is the tetramer. Each asymmetric unit contains one biologically-relevant tetramer.

serine protease, activated ketone inhibitor, pyrrolidine, CRA-29382, HYDROLASE