Crystal Structure of human cyclic GMP-AMP synthase in complex with 8-chloro-2-(2-hydroxyphenyl)quinoline-4-carboxylic acid

(2P)-8-chloro-2-(2-hydroxyphenyl)quinoline-4-carboxylic acid

Crystal Structure of a human cyclic GMP-AMP synthase complex

cGAMP synthase,cGAS,h-cGAS,2'3'-cGAMP synthase,Mab-21 domain-containing protein 1

10-12 mg/mL protein in 25 mM Tris/HCl pH7.5, 500mM NaCl, 2mM TCEP, supplemented with 10x molar excess of ligand and, if needed, with 10 mM MgCl2 and 5mM ATP, then mixed 1:1 with reservoir of the Procomplex screen. Several conditions resulted in crystals

rather high Rfree for this resolution, but ligand clearly defined by electron density;

NUCLEOTIDYLTRANSFERASE, CYCLIC GMP-AMP SYNTHASE, CGAS, DNA-BINDING, ACTIVATOR DNA, PATTERN RECOGNITION RECEPTOR, INNATE IMMUNE RESPONSE, VIRAL DNA RECOGNITION, TRANSFERASE