Crystal Structure of human cyclic GMP-AMP synthase in complex with 5-(4-fluorophenyl)-2-methylpyrazole-3-carboxylic acid

3-(4-fluorophenyl)-1-methyl-1H-pyrazole-5-carboxylic acid

Crystal Structure of a human cyclic GMP-AMP synthase complex

cGAMP synthase,cGAS,h-cGAS,2'3'-cGAMP synthase,Mab-21 domain-containing protein 1

10-12 mg/mL protein in 25 mM Tris/HCl pH7.5, 500mM NaCl, 2mM TCEP, supplemented with 10x molar excess of ligand and, if needed, with 10 mM MgCl2 and 5mM ATP, then mixed 1:1 with reservoir of the Procomplex screen. Several conditions resulted in crystals.

ligand well defined by electron density. 19F library NMR fragment hit

IMMUNE RESPONSE, TRANSFERASE, NTASE, DNA SENSOR