Cryo-EM structure of human p97 bound to UPCDC30245 inhibitor

2.3 A Resolution Cryo-Em Structure of Human P97 and Mechanism of Allosteric Inhibition

N-TERMINAL RESIDUES 21-200 DISORDERED. LINKER CONNECTING RESIDUES 707 TO 728 IS NOT IN THE MODEL. SUBMISSION BASED ON EXPERIMENTAL DATA FROM EMDB EMD-3295. (DEPOSITION ID: 14163).

25 MM TRIS, 150 MM NACL, 1 MM MGCL2, 1.0 MM TCEP

FULL-LENGTH HUMAN P97 BOUND TO UPCDC30245 INHIBITOR

VITRIFICATION 1 -- CRYOGEN- ETHANE, HUMIDITY- 100, TEMPERATURE- 90.15, INSTRUMENT- FEI VITROBOT MARK IV, METHOD- BLOT FOR 2.5 SECONDS BEFORE PLUNGING.

TRANSITIONAL ENDOPLASMIC RETICULUM ATPASE

TER ATPASE, 15S MG(2+)-ATPASE P97 SUBUNIT, VALOSIN-CONTAINING PROTEIN, VCP, P97 VCP TRANSITIONAL ENDOPLASMIC RETICULUM ATPASE

CRYO-EM STRUCTURE OF HUMAN P97 BOUND TO ADP

CRYO-EM STRUCTURE OF HUMAN P97 BOUND TO ATPGS ( CONFORMATION I)

CRYO-EM STRUCTURE OF HUMAN P97 BOUND TO ATPGS ( CONFORMATION II)

CRYO-EM STRUCTURE OF HUMAN P97 BOUND TO ATPGS ( CONFORMATION III)

TRANSITIONAL ENDOPLASMIC RETICULUM ATPASE (E.C.3.6.4.6)
HYDROLASE, SINGLE-PARTICLE, P97, AAA ATPASE