Structure of Transhydrogenase (dI.S138A.NADH)2(dIII.NADPH)1 asymmetric complex

1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE

NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE

The Role of Invariant Amino Acid Residues at the Hydride Transfer Site of Proton-translocating Transhydrogenase.

NAD(P) transhydrogenase subunit alpha part 1

Pyridine nucleotide transhydrogenase subunit alpha 1, Nicotinamide nucleotide transhydrogenase subunit alpha 1, Proton-translocating transhydrogenase component 1, dI

Pyridine nucleotide transhydrogenase subunit beta, Nicotinamide nucleotide transhydrogenase subunit beta, Proton-translocating transhydrogenase NADPH, -binding component, dIII

22-24% 8K-PEG, 60-140 mM (NH4)2SO4, 100 mM Mes, pH 6.0 and 10% glycerol in the presence of 50 mM NADH and 5 mM NADPH, VAPOR DIFFUSION, SITTING DROP, temperature 277K

LIGAND

NAD 500 IS ASSOCIATED WITH TRANSHYDROGENASE DI CHAIN A. NAD 600 IS ASSOCIATED WITH TRANSHYDROGENASE DI CHAIN B. NAP 300 IS ASSOCIATED WITH TRANSHYDROGENASE DIII CHAIN C.

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS

NAD(P) TRANSHYDROGENASE SUBUNITS, NADH, NADPH, OXIDOREDUCTASE