Structure of LH2 from Rps. acidophila crystallized in lipidic mesophases

(3E)-3,4-didehydro-1',2'-dihydro-psi,psi-caroten-1'-yl beta-D-glucopyranoside

Room to Move: Crystallizing Membrane Proteins in Swollen Lipidic Mesophases

Chirality errors at CA centers of several residues.

Light-harvesting protein B-800/850, alpha chain

Light-harvesting protein B-800/850, beta chain

200 microlitres of 40 %(w/w) protein, 0.05 %(w/v) LDAO, 20mM Tris/HCl and 60 %(w/w) monoolein mixed with 2 microlitres of precipitant: 20 %(w/v) pentaerythritol propoxylate, 0.1 M potassium formate, 0.1 M Tris/HCl, pH 8.0, Mesophase crystallization, lipidic cubic phase, temperature 293K

LH2 structure from crystallized in detergent at 2.5 Angstroms resoltion

LH2 structure from crystallized in detergent at 2.0 Angstroms resoltion

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS

Light-harvesting protein B-800/850, alpha chain, Light-harvesting protein B-800/850, beta chain

The biological assembly is a nonamer which is also the asymmetric unit of the cell.

Light Harvesting Complex B800-B850, Lipidic Mesophase, crystallization, cubic phase, sponge phase, LDAO, rhodopin glucoside carotenoid, MEMBRANE PROTEIN, PHOTOSYNTHESIS