Crystal structure of the bacteriophage Mu transpososome

The Mu transpososome structure sheds light on DDE recombinase evolution.

24-28% PEG400, 0.2 M magnesium chloride, 0.1 M HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 292K

(chain 'C' and ((resseq 2:17))) or (chain 'B' and ((resseq 88:165))) or (chain 'D' and ((resseq 41:54)))

(chain 'C' and ((resseq 18:25))) or (chain 'B' and ((resseq 178:242))) or (chain 'D' and ((resseq 31:40)))

(chain 'A' and ((resseq 88:165))) or (chain 'C' and ((resseq 26:38))) or (chain 'D' and ((resseq 20:30)))

(chain 'A' and ((resseq 178:247))) or (chain 'C' and ((resseq 39:51))) or (chain 'B' and ((resseq 568:605))) or (chain 'D' and ((resseq 7:19)))

(chain 'C' and ((resseq 52:69))) or (chain 'E' and ((resseq 2:14)))

RNaseH, DDE transposase, DNA BINDING PROTEIN-DNA complex

chain 'B' and (resseq 258:272 or resseq 276:285 or resseq 288:338 or resseq 364:367 or resseq 371:373 or resseq 426:472 or resseq 476:480 )

chain 'A' and (resseq 258:272 or resseq 276:285 or resseq 288:338 or resseq 364:367 or resseq 371:373 or resseq 426:472 or resseq 476:480 )

chain 'B' and (resseq 180:184 or resseq 188:189 or resseq 208:212 )

chain 'A' and (resseq 180:184 or resseq 188:189 or resseq 208:212 )

chain 'B' and (resseq 491:504 or resseq 508:545 or resseq 555:560 )

chain 'A' and (resseq 491:504 or resseq 508:545 or resseq 555:560 )

chain 'B' and (resseq 89:95 or resseq 99:101 or resseq 105:139 or resseq 143:145 or resseq 152:165 )

chain 'A' and (resseq 89:95 or resseq 99:101 or resseq 105:139 or resseq 143:145 or resseq 152:165 )