Crystal Structure of the Co2+2-Human Arginase I-AGPA Complex

Binding of the unreactive substrate analog L-2-amino-3-guanidinopropionic acid (dinor-L-arginine) to human arginase I.

Cryogenically cooled double crystal monochromater with horizontal focusing sagittal bend second mono crystal with 4:1 magnification ratio and vertically focusing mirror.

An unliganded Co2+2-HAI crystal was soaked in 20 mM AGPA, 5 mM CoCl2, 100 mM bicine (pH 8.5), 32% (v/v) Jeffamine ED-2001 for 18 hours, VAPOR DIFFUSION, HANGING DROP, temperature 298K

The same protein having the same weak inhibitor bound (AGPA); however, this protein has a Mn2+2-cluster.

The same protein except that it is unliganded and contains a Mn2+2-cluster as opposed to a Co2+2-cluster.

Arginase Fold, Hydrolase, Hydrolase-Hydrolase inhibitor complex