AlfA from B. subtilis plasmid pLS32 filament structure at 3.4 A

Cryo-EM reconstruction of AlfA fromBacillus subtilisreveals the structure of a simplified actin-like filament at 3.4- angstrom resolution.

20 mM Tris-HCl, 100 mM KCl, 2 mM TCEP, 1mM NaN3, pH 8.0, 5 mM ATP and 10 mM MgCl2 added to

plasmid segregation, bacterial cytoskeleton, cytomotive filament, VIRAL PROTEIN