Crystal structure of catalytically-active Streptococcus pyogenes CRISPR-Cas9 in complex with single-guided RNA and double-stranded DNA primed for target DNA cleavage

Structures of a CRISPR-Cas9 R-loop complex primed for DNA cleavage.

CRISPR-associated endonuclease Cas9/Csn1

DNA (5'-D(P*AP*TP*GP*AP*GP*AP*CP*GP*CP*TP*GP*GP*AP*GP*TP*AP*CP*AP*C)-3')

1.8 M ammonium sulfate, 100 mM Tris-HCl pH8.0 and 10 mM EDTA

RNA was prepared by in vitro transcription with T7 RNA polymerase

CRISPR-associated endonuclease Cas9/Csn1/RNA Complex

CRISPR, Cas9, R-loop, genome engineering, HYDROLASE-DNA-RNA complex