Two domain fragment of HIV-2 integrase in complex with LEDGF IBD

A novel co-crystal structure affords the design of gain-of-function lentiviral integrase mutants in the presence of modified PSIP1/LEDGF/p75

N-terminal and catalytic domains, UNP residues 1173-1380

LEDGF, Integrase binding domain, UNP residues 347-435

LEDGF, Lens epithelium-derived growth factor, Transcriptional coactivator p75/p52, Dense fine speckles 70 kDa protein, DFS 70, CLL-associated antigen KW-7

2.6M sodium acetate, 10mM MgCl2, 0.1M Bis-Tris Propane-HCl, pH7.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K

N-terminal and catalytic domains of HIV-1 integrase

Catalytic domain of HIV-1 integrase in complex with LEDGF IBD

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS, The structure was refined also with PHENIX
PDB ENTRY 2B4J (chains A and C), 1K6Y (chain A residue 1-43)
Integrase (E.C.3.4.23.47), PC4 and SFRS1-interacting protein
Protein-protein complex, AIDS, DNA integration, endonuclease, magnesium, metal-binding, multifunctional enzyme, nuclease, nucleotidyltransferase, nucleus, transferase, viral nucleoprotein, virion, DNA-binding, host-virus interaction, transcription, transcription regulation, zinc binding, HHCC motif, VIRAL PROTEIN, RECOMBINATION