Crystal structure of LkcE E64Q mutant in complex with LC-KA05

[(2~{S},5~{R},8~{S},11~{S})-1-[(2~{R},3~{R},5~{R},6~{S})-3,5-dimethyl-6-oxidanyl-4-oxidanylidene-oxan-2-yl]-5,11-dimethyl-8-oxidanyl-13-[[(2~{S})-2-oxidanylpropanoyl]amino]tridecan-2-yl] ethanoate

Insights into a dual function amide oxidase/macrocyclase from lankacidin biosynthesis.

16 % PEG 8000, 160 mM calcium acetate, Bis Tris 100 mM pH 6.5

6F32 is the structure of wild type LkcE with substrate analogues

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS U VALUES : REFINED INDIVIDUALLY

Amine oxydase, cyclase, Post-PKS enzyme, tayloring enzyme, FLAVOPROTEIN