Alignment of guide-target seed duplex within an argonaute silencing complex

Structure of an argonaute silencing complex with a seed-containing guide DNA and target RNA duplex.

DNA (5'-D(P*DTP*DGP*DAP*DGP*DGP*DTP*DAP*DGP*DTP*DAP*DGP*DGP*DTP*DTP*DGP*DTP*DA*DTP*DAP*DGP*DT)-3')

RNA (5'-R(*UP*AP*UP*AP*CP*AP*A*CP*UP*CP*AP*CP*UP*AP*CP*CP*UP*CP*GP*U)-3')

10% (v/v) PEG400, 50mM Mes 5.6, 0.1M KCl, 15 mM MgCl2, VAPOR DIFFUSION, HANGING DROP, temperature 308K

argonaute, protein-DNA-RNA-complex, NUCLEIC ACID BINDING PROTEIN-DNA-RNA COMPLEX