CRYSTAL STRUCTURE ANALYSIS OF N-ACETYLNEURAMINATE LYASE FROM HAEMOPHILUS INFLUENZAE: CRYSTAL FORM II IN COMPLEX WITH 4-OXO-SIALIC ACID

4,4,6,7,8,9-HEXAHYDROXY-5-METHYLCARBOXAMIDONONANOIC ACID

6,7,8,9-TETRAHYDROXY-5-METHYLCARBOXAMIDO-4-OXONONANOIC ACID

Active site modulation in the N-acetylneuraminate lyase sub-family as revealed by the structure of the inhibitor-complexed Haemophilus influenzae enzyme.

PEG 4000, sodium acetate, ammonium acetate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K

N-acetylneuraminate lyase from Escherichia coli Izard, T., Lawrence, M.C., Malby, R.L., Lilley, G.G., Colman, P.M.: The three-dimensional struture of N-acetylneuraminate lyase Escherichia coli. Structure (1994) 2, 361.

Structure and Mechanism of a Sub-Family of Enzymes Related to N-acetylneuraminate Lyase
Lawrence, M.C., Barbosa, J.A.R.G., Smith, B.J., Hall, N.E., Pilling, P.A., Ooi, H.C., Marcuccio, S.M.:
J. Mol. Biol. (1997) 266, 381-399

The biological assembly is a tetramer constructed from chains A and C generated by the two-fold.