CRYSTAL STRUCTURE OF TRYPANOSOMA BRUCEI ORNITHINE DECARBOXYLASE (ODC) COMPLEXED WITH PUTRESCINE, ODC'S REACTION PRODUCT.

Altering the reaction specificity of eukaryotic ornithine decarboxylase.

MODIFIED PET-11D PLASMID WITH HIS6-TAG, TEV PROTEASE SITE AND T7 PROMOTOR. HIS6-TAG WAS CLEAVED.

PEG 3350, sodium acetate, HEPES, DTT, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 289.0K

Native structure of ornithine decarboxylase from Trypanosoma brucei. 1QU4 contains an uncomplexed version of the same protein from the same species.

Structure of a K69A mutant of ornithine decarboxylase from Trypanosoma brucei complexed with DMO, a covalently-bound inhibitor. 2TOD contains a mutant of the same protein from the same species complexed with DMO, a covalently-bound inhibitor.

7ODC contains the structure of a homologue from Mus Musculus (not complexed).

beta-alpha-barrel, modified Greek key beta-sheet, LYASE