SigN RNA polymerase early-melted intermediate bound to full duplex DNA fragment dhsU36 (-12T)

A general mechanism for transcription bubble nucleation in bacteria.

Particle number and FSC resolution estimate of full map provided

dose-fractionation, 0.05 s/frame (=40 frames total)

Self-built glow discharge unit was evacuated using a vacuum pump prior to application of voltage (no pressure and/or voltage readings available)

NU refinement with global CTF and per-particle defocus refinement enabled

DNA-directed RNA polymerase subunit alpha

DNA-directed RNA polymerase subunit beta

DNA-directed RNA polymerase subunit beta'

DNA-directed RNA polymerase subunit omega

RNAP subunit alpha,RNA polymerase subunit alpha,Transcriptase subunit alpha

RNAP subunit beta,RNA polymerase subunit beta,Transcriptase subunit beta

RNAP subunit beta',RNA polymerase subunit beta',Transcriptase subunit beta'

RNAP omega subunit,RNA polymerase omega subunit,Transcriptase subunit omega

rpoB, groN, nitB, rif, ron, stl, stv, tabD, b3987, JW3950

National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)

promoter-bound, initiation, DNA melting, transcription bubble nucleation, TRANSCRIPTION, TRANSCRIPTION-DNA complex