Cryo-EM structure of Kap114 bound to H2A-H2B

Mechanism of RanGTP priming H2A-H2B release from Kap114 in an atypical RanGTP•Kap114•H2A-H2B complex.

20 mM Tris pH 7.5, 300 mM NaCl, 2 mM MgCl2, 1 mM TCEP, and 0.1% NP-40

Tris(2-carboxyethyl)phosphine hydrochloride

Complex of Kap114 bound to H2A-H2B dimer

114 kDa karyopherin,Karyopherin subunit beta-5,Karyopherin-114

Escherichia coli 'BL21-Gold(DE3)pLysS AG'

National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)

human importin homolog bound to X.l. H2A-H2B

Karyopherin Beta Nuclear Transport Histone Histone Chaperone, PROTEIN TRANSPORT-STRUCTURAL PROTEIN complex