Cryo EM structure of Vibrio cholerae NQR

Novel cofactor binding motifs, electron transfer and ion pumping mechanisms of the respiratory complex NQR

The model was refined by iterating between manual edits in Coot, molecular dynamics-guided edits using the ISOLDE ChimeraX package, and automated real-space refinement in Phenix. Models that had been automatically rebuilt using Rosetta were used as guides during manual refinement.

100 mM KCl, 1 mM EDTA, 50 mM HEPES, pH 7.0

Na(+)-translocating NADH-quinone reductase

Micrograph movies were summed and dose-weighted using the motion correction algorithm implemented in RELION.

50 e-/A2 fractionated over 40 movie frames

Gatan Solarus plasma cleaner operated at 20W and using ambient/room air

A 3.5 uL droplet of sample was applied to the grid surface and blotted for 4 s before plunging into liquid ethane.

Na(+)-translocating NADH-quinone reductase subunit A

Na(+)-translocating NADH-quinone reductase subunit B

Na(+)-translocating NADH-quinone reductase subunit C

Na(+)-translocating NADH-quinone reductase subunit D

Na(+)-translocating NADH-quinone reductase subunit E

Na(+)-translocating NADH-quinone reductase subunit F

Na(+)-NQR subunit A,Na(+)-translocating NQR subunit A,NQR complex subunit A,NQR-1 subunit A

Na(+)-NQR subunit B,Na(+)-translocating NQR subunit B,NQR complex subunit B,NQR-1 subunit B

Na(+)-NQR subunit C,Na(+)-translocating NQR subunit C,NQR complex subunit C,NQR-1 subunit C

Na(+)-NQR subunit D,Na(+)-translocating NQR subunit D,NQR complex subunit D,NQR-1 subunit D

Na(+)-NQR subunit E,Na(+)-translocating NQR subunit E,NQR complex subunit E,NQR-1 subunit E

Na(+)-NQR subunit F,Na(+)-translocating NQR subunit F,NQR complex subunit F,NQR-1 subunit F

National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)

complex, ELECTRON TRANSPORT, TRANSLOCASE