Designed pentafoil knot protein folded into a trefoil knot

0.2 M NaCl, 0.1 M Imidazole pH 8.0, 0.15 M Sodium phosphate monobasic, 1.35 M Potassium phosphate dibasic

National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)

de novo, knot, pentafoil, trefoil, DE NOVO PROTEIN