Crystal structure of AMPylated GRP78 with nucleotide

AMPylation targets the rate-limiting step of BiP's ATPase cycle for its functional inactivation.

5%PEG1000, 0.1M Na2HPO4-Citrate, PH4.2, 0.2M Li2SO4

5O4P contains the same protein, but purified differently and crystallized in a different condition.

The protein sample was shown as monomeric by gel filtration.

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS