Crystal structure of active site inhibited coagulation factor VIIA mutant in complex with soluble tissue factor

alpha-L-fucose; 6-deoxy-alpha-L-galactopyranose; L-fucose; fucose

Mechanism of the Ca2+-induced enhancement of the intrinsic factor VIIa activity

TF, Coagulation factor III, Thromboplastin

100mM NaCitrate, 16%(w/v) PEG4000, 12%(v/v) 1-propanol, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K

THE UNBOUND FORM OF THE INHIBITOR IS D-PHE-PHE-ARG-CHLOROMETHYLKETONE. UPON REACTION WITH PROTEIN IT FORMS TWO COVALENT BONDS: 1) A COVALENT BOND TO SER 344 H FORMING A HEMIKETAL AR7 AND 2) A COVALENT BOND TO NE2 OF HIS 193 H

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS

Serine protease, BLOOD CLOTTING, HYDROLASE-HYDROLASE INHIBITOR complex