Golgi alpha-Mannosidase II in complex with 5-substitued swainsonine analog: (5R)-5-[2'-oxo-2'-(4-methylphenyl)ethyl]-swainsonine

Structural Investigation of the Binding of 5-Substituted Swainsonine Analogues to Golgi alpha-Mannosidase II.

Alpha-mannosidase II, AMAN II, MAN II, Mannosyl-oligosaccharide 1,3-1,6-alpha-mannosidase, Golgi alpha-mannosidase II

PEG8000, Tris, pH 7, 2.5% MPD, VAPOR DIFFUSION, HANGING DROP, temperature 298K

dGMII + 5-thio-D-mannopyranosylamidinium salt

dGMII acid-base catalyst mutant D341N + mannose

dGMII_D204A with native substrate GlcNacMan5GlcNac

dGMII + (5R)-5-[2'-oxo-2'-(phenyl)ethyl]-swainsonine

dGMII + (5R)-5-[2'-oxo-2'-(4-tert-butylphenyl)ethyl]-swainsonine

dGMII + (5R)-5-[2'-(4-tert-butylphenyl)ethyl]-swainsonin

dGMII + (5S)-5-[2'-(4-tert-butylphenyl)ethyl]-swainsonine

dGMII + (5S)-5-[2'-oxo-2'-(4-tert-butylphenyl)ethyl]-swainsonine

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS

GH38 Glycosidase, Glycosidase, Golgi apparatus, Hydrolase, Membrane, Metal-binding, Signal-anchor, Transmembrane