PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER

Mutational Studies on Hslu and its Docking Mode with Hslv

The Structures of Hslu and the ATP-Dependent Protease Hslu-Hslv

Crystal Structure of Heat Shock Locus V (Hslv) from Escherichia Coli

Hslv-Hslu: A Novel ATP-Dependent Protease Complex in Escherichia Coli Related to the Eukaryotic Proteasome

Sequence Analysis of Four New Heat-Shock Genes Constituting the Hslts/Ibpab and Hslvu Operons in Escherichia Coli

20 MG/ML SOLUTION OF HSLU SUPPLEMENTED WITH 1 MM AMP-PNP IN BUFFER (20 MM TRIS/HCL, PH 7.5, 1 MM EDTA, 1 MM NAN3) MIXED IN 2:1 VOLUME RATIO WITH 16 MG/ML HSLV IN 300 MM NACL, 20 MM TRIS/HCL, PH 7.5, 1 MM EDTA, 1 MM NAN3. 0.002 ML RESERVOIR PLUS 0.002 ML PROTEIN SOLUTION EQUILIBRATED AGAINST 0.5 ML RESERVOIR SOLUTION. RESERVOIR CONTAINED 100 MM MES, PH 6.3 AND 2.0 M SODIUM ACETATE 0.4 MG/ML RESORUFIN-LABELLED CASEIN

CRYSTAL STRUCTURE OF HSLV (CLPQ) AT 3.8 ANGSTROMS RESOLUTION

ORTHORHOMBIC CRYSTAL FORM OF HEAT SHOCK LOCUS U (HSLU) FROM ESCHERICHIA COLI

TRIGONAL CRYSTAL FORM OF HEAT SHOCK LOCUS U (HSLU) FROM ESCHERICHIA COLI

SHEET
DETERMINATION METHOD: AUTHOR PROVIDED.

EDMAN-DEGRADATION HAS SHOWN THAT THE AMINO-TERMINAL METHIONINE IS CLEAVED IN HSLV TO EXPOSE A THREONINE RESIDUE THAT ACTS AS THE NUCLEOPHILE IN PROTEOLYSIS. FOR CONSISTENCY WITH THE PROTEASOME NUMBERING SCHEME, THIS THREONINE RESIDUE IS ASSIGNED SEQUENCE NUMBER 1. THE FOLLOWING RESIDUES ARE NUMBERED CONSECUTIVELY, UNLIKE IN ENTRY 1NED FOR HSLV WHERE THE NUMBERING SCHEME HAS BEEN CHOSEN TO EMPHASIZE THE SIMILARITY OF HSLV WITH THE BETA-SUBUNITS OF 20S PROTEASOMES. AN ENGINEERED VARIANT OF HSLV WITH THE CARBOXY-TERMINAL TAG EFHHHHHH WAS USED FOR CRYSTALLIZATION. AS THE TAG RESIDUES AND THE LAST TWO RESIDUES OF THE WILD TYPE SEQUENCE ARE NOT VISIBLE IN THE ELECTRON DENSITY, THEY HAVE BEEN OMITTED FROM THE MODEL.

ZERO OCCUPANCY COORDINATES IN I- DOMAIN ARE TAKEN FROM THOSE OF TRIGONAL HSLU MODEL (1DO2) THE ELECTRON DENSITY OF RESIDUES FROM 175 - 209 IN HSLU MODEL (CHAIN E AND F) IS COMPLETELY DISORDERED THE ELECTRON DENSITY OF CORE REGION (RESIDUE FROM 89 - 92) IN HSLU (CHAIN E AND F) WAS NOT CLEAR AND MANY ATOMS IN THIS REGION HAVE ZERO OCCUPANCY.

HEAT SHOCK PROTEIN HSLV, HEAT SHOCK PROTEIN HSLU

CHAPERONE, HSLVU, CLPQY, AAA-ATPASE, ATP-DEPENDENT PROTEOLYSIS, PROTEASOME