Cryo-EM structure of the flagellar rod with partial hook from Salmonella

Structural basis of assembly and torque transmission of the bacterial flagellar motor.

tris(hydroxymethyl)aminomethane hydrochloride-hydrochloride

Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

Flagellar hook-basal body complex protein FliE

Ministry of Science and Technology (MoST, China)

National Natural Science Foundation of China (NSFC)

Structure of the Salmonella flagella rod with hook and partial export apparatus

The complex was purified from the natural source and the sequence of Flagellar M-ring protein is listed below:
MSATASTATQPKPLEWLNRLRANPRIPLIVAGSAAVAIVVAMVLWAKTPDYRTLFSNLSD
QDGGAIVAQLTQMNIPYRFANGSGAIEVPADKVHELRLRLAQQGLPKGGAVGFELLDQEK
FGISQFSEQVNYQRALEGELARTIETLGPVKSARVHLAMPKPSLFVREQKSPSASVTVTL
EPGRALDEGQISAVVHLVSSAVAGLPPGNVTLVDQSGHLLTQSNTSGRDLNDAQLKFAND
VESRIQRRIEAILSPIVGNGNVHAQVTAQLDFANKEQTEEHYSPNGDASKATLRSRQLNI
SEQVGAGYPGGVPGALSNQPAPPNEAPIATPPTNQQNAQNTPQTSTSTNSNSAGPRSTQR
NETSNYEVDRTIRHTKMNVGDIERLSVAVVVNYKTLADGKPLPLTADQMKQIEDLTREAM
GFSDKRGDTLNVVNSPFSAVDNTGGELPFWQQQSFIDQLLAAGRWLLVLVVAWILWRKAV
RPQLTRRVEEAKAAQEQAQVRQETEEAVEVRLSKDEQLQQRRANQRLGAEVMSQRIREMS
DNDPRVVALVIRQWMSNDHE.
Residues 311-331 (chains 5/6/7/8/9) could be precisely identified. But the residues in chains 0/1/2/3/4 could not be precisely built due to their low densities, and the model of L2 was built as UNK. The fragment likely contains the residues 'TNQQNAQNTPQTSTS' (333-347) according to its relative position to other fragment (residues 311-331).

Flagellar hook protein FlgE, Flagellar basal-body rod protein FlgG, Flagellar basal-body rod protein FlgF