Crystal structure of Snx9PX-BAR (230-595), H32

Structure and plasticity of endophilin and sorting nexin 9.

CHIRALITY ERRORS AT RESIDUES A503, A592, A594, C266, C284, C310, C312, C314.

SH3 and PX domain-containing protein 1, Protein SDP1, SH3 and PX domain-containing protein 3A

1.8M Ammonium sulfate, 0.1M Tris-HCl pH 8.5, 5% PEG 400, 0.05M MgSO4, 15% Xylitol, VAPOR DIFFUSION, HANGING DROP, temperature 293.15K

The same protein at high resolution and in space group C2221

STRUCTURE WAS SOLVED BY MOLECULAR REPLACEMENT AND NOT REFINED DUE TO VERY LOW RESOLUTION OF THE DATA COLLECTED. THE ONLY MODIFICATION PERFORMED TO THE OBTAINED MODEL FROM MOLECULAR REPLACEMENT WAS SOME RIGID BODY ADJUSTMENT OF HELICAL TIPS AND DOMAINS (RESIDUES 507-548, RESIDUES 230-390, RESIDUES 391-595) ACCORDING TO THE ELECTRON DENSITY. AUTHORS DID NOT HAVE ENOUGH DATA TO SEE THE SIDE CHAIN POSITIONS. THE CLASHES BETWEEN SIDE CHAINS OF SURFACE RESIDUES WERE REMOVED USING ALTERNATIVE ROTAMER CONFORMATION.

3-helix-bundle, BAR domain, PX domain, Phosphoprotein, Protein transport, SH3 domain, Transport, TRANSPORT PROTEIN