Succinyl-CoA:acetate CoA-transferase (AarCH6) bound to acetate and the CoA analogue 3'-phosphoadenosine 5'-(O-(N-propyl-R-pantothenamide))pyrophosphate (MX)

[[(2R,3S,4R,5R)-5-(6-aminopurin-9-yl)-4-oxidanyl-3-phosphonooxy-oxolan-2-yl]methoxy-oxidanyl-phosphoryl] [(3R)-2,2-dimethyl-3-oxidanyl-4-oxidanylidene-4-[[3-oxidanylidene-3-(propylamino)propyl]amino]butyl] hydrogen phosphate

Functional Dissection of the Bipartite Active Site of the Class I Coenzyme A (CoA)-Transferase Succinyl-CoA:Acetate CoA-Transferase.

Crystal structures of Acetobacter aceti succinyl-coenzyme A (CoA):acetate CoA-transferase reveal specificity determinants and illustrate the mechanism used by class I CoA-transferases.

0.9 M sodium citrate, 0.1 M imidazole, 25 mM 2-mercaptoethanol, 3 mM MX; drops adjusted to 50 mM sodium acetate (pH 8.2 stock) 3 d prior to cryoprotection

Same protein bound to dethiaacetyl-CoA (AcMX) derived acetate and CoA analogue, with one acetylglutamyl anhydride adduct.

Tricarboxylic acid cycle, Transferase, Acidophile, coenzyme analogue