Crystal structure of human ALDH2 in complex with NAD+ and PEG MME 550

protein conditions: 8 mg/ml recombinant ALDH2 in 20 mM Tris-HCl, pH 8.0, 50 mM NaCl, 1 mM DTT, supplemented with 2-4% v/v DMSO
well conditions: 100 mM sodium citrate, pH 5.0-5.6, 22-26% w/v PEG MME 550 550
hanging drops were set with a 1.5:1 protein:well ratio

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS U VALUES : REFINED INDIVIDUALLY

ALDH2, aldehyde dehydrogenase, mitochondrial, OXIDOREDUCTASE