A designed ankyrin repeat protein selected to bind to tubulin caps the microtubule plus end.

Designed ankyrin repeat protein (DARPIN) D1

PEG 6K, 0.05M KCl, 0.01 M MGCL2, 0.2M LiCl, 0.01 M HEPES pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 292K

AUTHOR STATES THAT FOR ALPHA-TUBULIN (CHAIN A AND CHAIN C), THE BOVINE BRAIN TUBULIN ALPHA 1B SEQUENCE (UNP81947) WAS USED FOR REFINEMENT BECAUSE THE SEQUENCE OF OVINE BRAIN TUBULIN IS NOT AVAILABLE. FOR BETA-TUBULIN (CHAIN B AND CHAIN D), THERE ARE THREE MAJOR ISOTYPES EXPRESSED IN THE BRAIN. THEY USED THE BETA 2B ISOTYPE SEQUENCE (NCBI NP_ 999 001003900.1)

Tubulin alpha chain, Tubulin beta chain, Designed ankyrin repeat protein (DARPIN) D1

ALPHA-TUBULIN, BETA-TUBULIN, GTPASE, MICROTUBULE, DARPin, SUBTILISIN, TUBULIN, TUBULIN DIGESTED WITH SUBTILISIN, CELL CYCLE

chain 'B' and (resseq 1:42 or resseq 45: 54 or resseq 62:360 or resseq 369:441 ) and (not element H)

chain 'D' and (resseq 1:42 or resseq 45: 54 or resseq 62:360 or resseq 369:441 ) and (not element H)

chain 'A' and (resseq 2:37 or resseq 47: 279 or resseq 285:346 or resseq 350:437 ) and (not element H)

chain 'C' and (resseq 2:37 or resseq 47: 279 or resseq 285:346 or resseq 350:437 ) and (not element H)

chain 'E' and (resseq 13:167 ) and (not element H)

chain 'F' and (resseq 13:167 ) and (not element H)